BRADYKININ POTENTIATES ACETYLCHOLINE-INDUCED RESPONSES IN NATIVE ENDOTHELIAL-CELLS FROM RABBIT AORTA

被引：10
|
作者：
WANG, XD [1 ]
CHU, W [1 ]
LAU, F [1 ]
VANBREEMEN, C [1 ]
机构：
[1] UNIV BRITISH COLUMBIA,FAC MED,DEPT PHARMACOL & THERAPEUT,VANCOUVER,BC V6T 1G3,CANADA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 213卷 / 03期
关键词：
D O I：
10.1006/bbrc.1995.2235
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Membrane potential and intracellular [Ca2+] were measured in freshly isolated rabbit aortic endothelial cells. Acetylcholine (10 mu M) induced transient membrane hyperpolarization and increased [Ca2+](i). Bradykinin (4 mu M) had no direct effect on membrane potential or [Ca2+](i) on its own, but potentiated the subsequent ACh response. It changed the usual transient ACh response to a maintained hyperpolarization. The maintained hyperpolarization was blocked by NiCl2 and TEA, indicating an involvement of receptor-operated-Ca2+ channel and Ca2+-dependent K+-channel activity. This potentiating effect of bradykinin in isolated cells was confirmed in organ bath studies using isolated aortic rings. (C) 1995 Academic Press, Inc.
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页码：1061 / 1067
页数：7
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