Cucumber mosaic virus (CMV) RNA-dependent RNA polymerase (RdRp) was purified form CMV-infected tobacco. The purified enzyme is completely dependent on exogenous template. The enzyme utilizes a variety of viral RNAs and CMV satellite RNA as template for minus-strand synthesis. Cellular RNAs are not used as templates. Ribosomal RNA inhibits the viral RNA synthesis by the CMV RdRp.
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Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
Babraham Inst, Cambridge CB22 3AT, EnglandUniv Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
Stubbs, Thomas M.
te Velthuis, Aartjan J. W.
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Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, EnglandUniv Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
机构:
Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R ChinaWuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R China
Lin, Meijuan
Ye, Shan
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Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R ChinaWuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R China
Ye, Shan
Xiong, Yi
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Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R ChinaWuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R China
Xiong, Yi
Cai, Dawei
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Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R ChinaWuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R China
Cai, Dawei
Zhang, Jiamin
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Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R ChinaWuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R China
Zhang, Jiamin
Hu, Yuanyang
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Wuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R ChinaWuhan Univ, State Key Lab Virol, Coll Life Sci, Wuhan 430072, Hubei Province, Peoples R China