CHARACTERIZATION OF CUCUMBER MOSAIC-VIRUS RNA-DEPENDENT RNA-POLYMERASE

被引:23
|
作者
QUADT, R
JASPARS, EMJ
机构
[1] Department of Biochemistry, Gorlacus Laboratories, Leiden University, 2300 RA Leiden
关键词
CUCUMBER MOSAIC VIRUS; RNA POLYMERASE; SATELLITE RNA; CELLULAR RNA;
D O I
10.1016/0014-5793(91)80166-Z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cucumber mosaic virus (CMV) RNA-dependent RNA polymerase (RdRp) was purified form CMV-infected tobacco. The purified enzyme is completely dependent on exogenous template. The enzyme utilizes a variety of viral RNAs and CMV satellite RNA as template for minus-strand synthesis. Cellular RNAs are not used as templates. Ribosomal RNA inhibits the viral RNA synthesis by the CMV RdRp.
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页码:273 / 276
页数:4
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