INTRAMOLECULAR DG.DG.DC TRIPLEX DETECTED IN ESCHERICHIA-COLI-CELLS

被引：60
|
作者：
KOHWI, Y [1 ]
MALKHOSYAN, SR [1 ]
KOHWISHIGEMATSU, T [1 ]
机构：
[1] LA JOLLA CANC RES FDN,10901 N TORREY PINES RD,LA JOLLA,CA 92037
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 223卷 / 04期
关键词：
CHLOROACETALDEHYDE; DG.DG.DC TRIPLEX; NON-B-DNA STRUCTURE; ESCHERICHIA-COLI CELLS; INSITU CHEMICAL PROBING;
D O I：
10.1016/0022-2836(92)90242-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The formation of an intramolecular dG·dG·dC triplex in Escherichia coli cells is demonstrated at single-base resolution. The intramolecular dG·dG·dC triplex structure was probed in situ for E. coli cells containing plasmid DNAs with varying lengths of poly(dG)·poly(dC) tracts employing chloroacetaldehyde. This chemical probe reacts specifically with unpaired DNA bases. The triplex structure formed with the poly(dG)·poly(dC) tracts of 35 and 44 base-pairs, but not with 25 base-pairs. The triplex was detected only one to two hours after the chloramphenicol treatment: the period at which the extracted plasmid DNA revealed the maximal superhelical density. © 1992.
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页码：817 / 822
页数：6
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