INTRAMOLECULAR DG.DG.DC TRIPLEX DETECTED IN ESCHERICHIA-COLI-CELLS

被引：60
|
作者：
KOHWI, Y [1 ]
MALKHOSYAN, SR [1 ]
KOHWISHIGEMATSU, T [1 ]
机构：
[1] LA JOLLA CANC RES FDN,10901 N TORREY PINES RD,LA JOLLA,CA 92037
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 223卷 / 04期
关键词：
CHLOROACETALDEHYDE; DG.DG.DC TRIPLEX; NON-B-DNA STRUCTURE; ESCHERICHIA-COLI CELLS; INSITU CHEMICAL PROBING;
D O I：
10.1016/0022-2836(92)90242-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The formation of an intramolecular dG·dG·dC triplex in Escherichia coli cells is demonstrated at single-base resolution. The intramolecular dG·dG·dC triplex structure was probed in situ for E. coli cells containing plasmid DNAs with varying lengths of poly(dG)·poly(dC) tracts employing chloroacetaldehyde. This chemical probe reacts specifically with unpaired DNA bases. The triplex structure formed with the poly(dG)·poly(dC) tracts of 35 and 44 base-pairs, but not with 25 base-pairs. The triplex was detected only one to two hours after the chloramphenicol treatment: the period at which the extracted plasmid DNA revealed the maximal superhelical density. © 1992.
引用
收藏
页码：817 / 822
页数：6
相关论文
共 50 条
  • [21] OBSERVATION OF THE HYDRATION-DEPENDENT CONFORMATION OF THE (DG)(20)CENTER-DOT(DG)(20)(DC)(20) OLIGONUCLEOTIDE TRIPLEX USING FTIR SPECTROSCOPY
    WHITE, AP
    POWELL, JW
    BIOCHEMISTRY, 1995, 34 (04) : 1137 - 1142
  • [22] Studies on the replication of the ring opened formamidopyrimidine, Fapy•dG in Escherichia coli
    Patro, Jennifer N.
    Wiederholt, Carissa J.
    Jiang, Yu Lin
    Delaney, James C.
    Essigmann, John M.
    Greenberg, Marc M.
    BIOCHEMISTRY, 2007, 46 (35) : 10202 - 10212
  • [23] EFFECTS OF TEMPERATURE ON THE SIZE AND SHAPE OF ESCHERICHIA-COLI-CELLS
    TRUEBA, FJ
    VANSPRONSEN, EA
    TRAAS, J
    WOLDRINGH, CL
    ARCHIVES OF MICROBIOLOGY, 1982, 131 (03) : 235 - 240
  • [24] MECHANISM OF SENSITIVITY TO PHLEOMYCIN IN GROWING ESCHERICHIA-COLI-CELLS
    SLEIGH, MJ
    GRIGG, GW
    BIOCHEMICAL JOURNAL, 1976, 155 (01) : 87 - 99
  • [25] SENSIBILIZATION OF ESCHERICHIA-COLI-CELLS BY CHOLESTEROL INCORPORATED INTO THEIR MEMBRANE
    BRESLER, SE
    ROZENBERG, OA
    NOSKIN, LA
    STEPANOVA, IM
    BEKETOVA, AG
    LOSHAKOVA, LV
    KOVALEVA, IG
    DOKLADY AKADEMII NAUK SSSR, 1984, 274 (02): : 445 - 448
  • [26] SITE OF RIBOSOME DEGRADATION IN STARVED ESCHERICHIA-COLI-CELLS
    KAPLAN, R
    HARTSTEIN, E
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1976, 251 (04) : 1147 - 1153
  • [27] TRANSPOSITION OF IS-ELEMENTS IN RESTING ESCHERICHIA-COLI-CELLS
    RAABE, T
    SENGSTAG, C
    MEYER, J
    ARBER, W
    EXPERIENTIA, 1986, 42 (06): : 692 - 692
  • [28] THE UPTAKE AND ACYLATION OF EXOGENOUS LYSOPHOSPHATIDYLETHANOLAMINE BY ESCHERICHIA-COLI-CELLS
    HELLION, P
    LANDRY, F
    SUBBAIAH, PV
    PROULX, P
    CANADIAN JOURNAL OF BIOCHEMISTRY, 1980, 58 (12): : 1381 - 1386
  • [29] CHARACTERIZATION OF THE PHOSPHOPROTEINS OF ESCHERICHIA-COLI-CELLS BY ELECTROPHORETIC ANALYSIS
    CORTAY, JC
    RIEUL, C
    DUCLOS, B
    COZZONE, AJ
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 159 (02): : 227 - 237
  • [30] PROBES OF MEMBRANE-POTENTIAL IN ESCHERICHIA-COLI-CELLS
    GHAZI, A
    SCHECHTER, E
    LETELLIER, L
    LABEDAN, B
    FEBS LETTERS, 1981, 125 (02) : 197 - 200
12345