Comparison of the Results of PCR Analysis of Gene Expression in Breast Cancer Tissue Specimens Stabilized in Formalin and RNAlater

被引：0

作者：

N. A. Khaustova

D. S. Makeeva

O. V. Kondrashina

N. N. Fedotov

I. N. Nechaev

N. A. Savelov

V. N. Grinevich

D. V. Maltseva

V. V. Galatenko

M. Yu. Shkurnikov

机构：

[1] BioClinicum Center,Research Institute of Pathology and Pathophysiology

[2] M. V. Lomonosov Moscow State University,undefined

[3] Russian Academy of Medical Sciences,undefined

[4] Moscow Municipal Oncologic Hospital No. 62,undefined

[5] Department of Health of Moscow,undefined

来源：

Bulletin of Experimental Biology and Medicine | 2014年 / 156卷

关键词：

real-time PCR; gene expression; formalin fixation; RNAlater; breast cancer;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.

引用

页码：486 / 490

页数：4

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