Comparison of the Results of PCR Analysis of Gene Expression in Breast Cancer Tissue Specimens Stabilized in Formalin and RNAlater

被引：0

作者：

N. A. Khaustova

D. S. Makeeva

O. V. Kondrashina

N. N. Fedotov

I. N. Nechaev

N. A. Savelov

V. N. Grinevich

D. V. Maltseva

V. V. Galatenko

M. Yu. Shkurnikov

机构：

[1] BioClinicum Center,Research Institute of Pathology and Pathophysiology

[2] M. V. Lomonosov Moscow State University,undefined

[3] Russian Academy of Medical Sciences,undefined

[4] Moscow Municipal Oncologic Hospital No. 62,undefined

[5] Department of Health of Moscow,undefined

来源：

Bulletin of Experimental Biology and Medicine | 2014年 / 156卷

关键词：

real-time PCR; gene expression; formalin fixation; RNAlater; breast cancer;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.

引用

页码：486 / 490

页数：4

共 50 条

[31] Methods for evaluating HER2 status in breast cancer: comparison of IHC, FISH, and real-time PCR analysis of formalin-fixed paraffin-embedded tissue
Olsson, Hans
Jansson, Agneta
Holmlund, Birgitta
Gunnarsson, Cecilia
PATHOLOGY AND LABORATORY MEDICINE INTERNATIONAL, 2013, 5 : 31 - 37
[32] A focused, PCR based gene expression signature to refine grade in breast cancer
Szasz, A.
Tokes, A. M.
Szittya, L.
Baranyak, Z. S.
Szekely, B.
Eklund, A.
Li, Q.
Swanton, C.
Szallasi, Z.
Kulka, J.
EJC SUPPLEMENTS, 2010, 8 (03): : 226 - 226
[33] Application of multiplex PCR with histopathologic features for detection of familial breast cancer in formalin-fixed, paraffin-embedded histological specimens
Rassi, H.
Gorovenko, N. G.
Houshmand, M.
Podolskaya, S. V.
Hashemi, M.
Majidzadeh, K.
Akbari, M. H. Hosseini
Panahi, M. Shafa Shariat
CYTOLOGY AND GENETICS, 2008, 42 (02) : 120 - 126
[34] Application of multiplex PCR with histopathologic features for detection of familial breast cancer in formalin-fixed, paraffin-embedded histological specimens
H. Rassi
N. G. Gorovenko
M. Houshmand
S. V. Podolskaya
M. Hashemi
K. Majidzadeh
M. H. Hosseini Akbari
M. Shafa Shariat Panahi
Cytology and Genetics, 2008, 42
[35] MiRNA expression profiling and validation in formalin-fixed paraffin-embedded breast cancer tissue
Zhao, Yingchun
Xiao, Jing
Zhang, Hengwei
Cao, Rui
Deng, Caishu
Gatalica, Zoran
Silva, Ed
Recker, Robert
Xiao, Gary
CANCER RESEARCH, 2009, 69
[36] Gene Expression Profiling of Formalin-Fixed, Paraffin-Embedded (FFPE) Breast Cancer Samples and Analysis of Intrinsic Subtypes
Baumbach, L. L.
Gomez, C.
Yan, J.
Halsey, T.
Ahearn, M. E.
Jorda, M.
Kennedy, R.
ODonnel, J.
McDyer, F.
Deharo, S.
Pegram, M.
CANCER RESEARCH, 2010, 70
[37] Serial analysis of gene expression in breast cancer cells
Aldaz, CM
Bednarek, A
Charpentier, A
MacLeod, M
Hawkins, K
Laflin, K
HORMONAL CARCINOGENESIS III, 2001, : 113 - 123
[38] Effects of Different Tissue Microenvironments on Gene Expression in Breast Cancer Cells
Rondeau, Gaelle
Abedinpour, Parisa
Desai, Prerak
Baron, Veronique T.
Borgstrom, Per
Welsh, John
PLOS ONE, 2014, 9 (07):
[39] An investigation of gene expression in human breast cancer using tissue microarray
Gabrovska, P.
Smith, R.
Griffiths, L.
BREAST, 2008, 17 (02): : 208 - 209
[40] Quantitative RT-PCR Analysis of Estrogen Receptor Gene Expression in Laser Microdissected Prostate Cancer Tissue
Walton, Thomas J.
Li, Geng
McCulloch, Thomas A.
Seth, Rashmi
Powe, Desmond G.
Bishop, Michael C.
Rees, Robert C.
PROSTATE, 2009, 69 (08): : 810 - 819

← 1 2 3 4 5 →