Comparison of the Results of PCR Analysis of Gene Expression in Breast Cancer Tissue Specimens Stabilized in Formalin and RNAlater

被引：0
|
作者：
N. A. Khaustova
D. S. Makeeva
O. V. Kondrashina
N. N. Fedotov
I. N. Nechaev
N. A. Savelov
V. N. Grinevich
D. V. Maltseva
V. V. Galatenko
M. Yu. Shkurnikov
机构：
[1] BioClinicum Center,Research Institute of Pathology and Pathophysiology
[2] M. V. Lomonosov Moscow State University,undefined
[3] Russian Academy of Medical Sciences,undefined
[4] Moscow Municipal Oncologic Hospital No. 62,undefined
[5] Department of Health of Moscow,undefined
来源：
Bulletin of Experimental Biology and Medicine | 2014年 / 156卷
关键词：
real-time PCR; gene expression; formalin fixation; RNAlater; breast cancer;
D O I：
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中图分类号：
学科分类号：
摘要：
We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.
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页码：486 / 490
页数：4
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