Nm-seq maps 2′-O-methylation sites in human mRNA with base precision

被引:0
|
作者
Qing Dai
Sharon Moshitch-Moshkovitz
Dali Han
Nitzan Kol
Ninette Amariglio
Gideon Rechavi
Dan Dominissini
Chuan He
机构
[1] The University of Chicago,Department of Chemistry
[2] Howard Hughes Medical Institute,Department of Biochemistry and Molecular Biology
[3] The University of Chicago,undefined
[4] Cancer Research Center,undefined
[5] Chaim Sheba Medical Center,undefined
[6] Wohl Centre for Translational Medicine,undefined
[7] Chaim Sheba Medical Center,undefined
[8] The Mina and Everard Goodman Faculty of Life Sciences,undefined
[9] Bar-Ilan University,undefined
[10] Sackler School of Medicine,undefined
[11] Tel Aviv University,undefined
[12] The University of Chicago,undefined
[13] Institute for Biophysical Dynamics,undefined
[14] The University of Chicago,undefined
来源
Nature Methods | 2017年 / 14卷
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学科分类号
摘要
Iterative oxidation, elimination and dephosphorylation steps remove nucleotides from the 3′ end of RNA until a 2′-O-methylated ribose that cannot be oxidized is encountered and brings the process to a stop. High-throughput sequencing of these fragments exposes 2′-O-methyl sites at base resolution.
引用
收藏
页码:695 / 698
页数:3
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