Nm-seq maps 2′-O-methytation sites in human mRNA with base precision

被引：205

作者：

Dai, Qing ^{[1
,2
]}

Moshitch-Moshkovitz, Sharon ^{[3
,4
]}

Han, Dali ^{[1
,2
]}

Kol, Nitzan ^{[3
]}

Amariglio, Ninette ^{[3
,4
,5
]}

Rechavi, Gideon ^{[3
,4
,6
]}

Dominissini, Dan ^{[3
,4
,6
]}

He, Chuan ^{[1
,2
,7
,8
]}

机构：

[1] Univ Chicago, Dept Chem, 5735 S Ellis Ave, Chicago, IL 60637 USA

[2] Univ Chicago, Howard Hughes Med Inst, 5841 S Maryland Ave, Chicago, IL 60637 USA

[3] Chaim Sheba Med Ctr, Canc Res Ctr, Tel Hashomer, Israel

[4] Chaim Sheba Med Ctr, Wohl Ctr Translat Med, Tel Hashomer, Israel

[5] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, Ramat Gan, Israel

[6] Tel Aviv Univ, Sackler Sch Med, Tel Aviv, Israel

[7] Univ Chicago, Dept Biochem & Mol Biol, 920 E 58th St, Chicago, IL 60637 USA

[8] Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA

来源：

NATURE METHODS | 2017年 / 14卷 / 07期

基金：

美国国家卫生研究院;

关键词：

RIBOSOMAL-RNA; METHYLATION; CAP; THERMODYNAMICS; TRANSLATION; ELIMINATION; BIOGENESIS; COMPLEXES; PROTEINS; SEQUENCE;

D O I：

10.1038/nmeth.4294

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

引用

页码：695 / +

页数：6

共 6 条

[1] Nm-seq maps 2′-O-methylation sites in human mRNA with base precision
Qing Dai
Sharon Moshitch-Moshkovitz
Dali Han
Nitzan Kol
Ninette Amariglio
Gideon Rechavi
Dan Dominissini
Chuan He
Nature Methods, 2017, 14 : 695 - 698
[2] Correction: Corrigendum: Nm-seq maps 2′-O-methylation sites in human mRNA with base precision
Qing Dai
Sharon Moshitch-Moshkovitz
Dali Han
Nitzan Kol
Ninette Amariglio
Gideon Rechavi
Dan Dominissini
Chuan He
Nature Methods, 2018, 15 : 226 - 227
[3] Nm-seq maps 2′-O-methylation sites in human mRNA with base precision (vol 14, pg 695, 2017)
Dai, Qing
Moshitch-Moshkovitz, Sharon
Han, Dali
Kol, Nitzan
Amariglio, Ninette
Rechavi, Gideon
Dominissini, Dan
He, Chuan
NATURE METHODS, 2018, 15 (03) : 226 - 227
[4] Single base resolution mapping of 2′-O-methylation sites in human mRNA and in 3′ terminal ends of small RNAs
Hsu, Phillip J.
Fei, Qili
Dai, Qing
Shi, Hailing
Dominissini, Dan
Ma, Lijia
He, Chuan
METHODS, 2019, 156 : 85 - 90
[5] Nm-Mut-seq: a base-resolution quantitative method for mapping transcriptome-wide 2′-O-methylation
Chen, Li
Zhang, Li-Sheng
Ye, Chang
Zhou, Huiqing
Liu, Bei
Gao, Boyang
Deng, Zixin
Zhao, Changming
He, Chuan
Dickinson, Bryan C.
CELL RESEARCH, 2023, 33 (09) : 727 - 730
[6] Nm-Mut-seq: a base-resolution quantitative method for mapping transcriptome-wide 2′-O-methylation
Li Chen
Li-Sheng Zhang
Chang Ye
Huiqing Zhou
Bei Liu
Boyang Gao
Zixin Deng
Changming Zhao
Chuan He
Bryan C. Dickinson
Cell Research, 2023, 33 : 727 - 730

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