The main aim of this paper is to define and investigate more general multiple Charlier polynomials on the linear lattice ωN={0,ω,2ω,…}\documentclass[12pt]{minimal}
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\begin{document}$\omega \mathbb{N} = \{ 0,\omega ,2\omega ,\ldots \} $\end{document}, ω∈R\documentclass[12pt]{minimal}
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\begin{document}$\omega \in \mathbb{R}$\end{document}. We call these polynomials ω-multiple Charlier polynomials. Some of their properties, such as the raising operator, the Rodrigues formula, an explicit representation and a generating function are obtained. Also an (r+1)\documentclass[12pt]{minimal}
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\begin{document}$( r+1 )$\end{document}th order difference equation is given. As an example we consider the case ω=32\documentclass[12pt]{minimal}
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\begin{document}$\omega =\frac{3}{2}$\end{document} and define 32\documentclass[12pt]{minimal}
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\begin{document}$\frac{3}{2}$\end{document}-multiple Charlier polynomials. It is also mentioned that, in the case ω=1\documentclass[12pt]{minimal}
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\begin{document}$\omega =1$\end{document}, the obtained results coincide with the existing results of multiple Charlier polynomials.