Enzyme mass-transfer coefficient in aqueous two-phase systems using static mixer extraction column

被引：0
|
作者：
K. Rostami
M. Alamshahi
机构：
[1] No. 71 Forsat Street,
[2] Biotechnology Center,undefined
[3] Iranian Research Organization for Science and Technology,undefined
[4] Tehran-15819,undefined
[5] Iran,undefined
来源：
Bioprocess and Biosystems Engineering | 2002年 / 25卷
关键词：
Enzyme mass transfer Static mixer Aqueous two-phase system;
D O I：
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中图分类号：
学科分类号：
摘要：
Recent technical advances in aqueous two-phase systems (ATPS) have made this a sound technique for the extraction of biomacromolecules. The extraction of α-amylase was investigated using aqueous two-phase systems formed by sodium sulphate–polyethylene glycol (PEG) in water in a 47-mm inner diameter spray column packed with three types of static mixers. The effects of dispersed-phase flow rate, phase composition, column height and diameter were studied. The extraction column was operated in a semi-batch manner. It was found that the hold-up and volumetric mass transfer coefficients increased with an increase in dispersed (PEG-rich) phase velocity and decreased with increasing phase composition. Empirical correlations were developed for fractional dispersed-phase hold-up and volumetric mass transfer coefficients.
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页码：169 / 178
页数：9
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