Optimized localization analysis for single-molecule tracking and super-resolution microscopy

被引：0
|
作者：
Mortensen K.I. [1 ]
Churchman L.S. [1 ,2 ,3 ]
Spudich J.A. [2 ]
Flyvbjerg H. [1 ]
机构：
[1] Department of Micro-and Nanotechnology, Technical University of Denmark, Kongens Lyngby
[2] Department of Biochemistry, Stanford University School of Medicine, Stanford, CA
[3] Department of Physics, Stanford University School of Medicine, Stanford, CA
来源：
Nature Methods | 2010年 / 7卷 / 5期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1038/nmeth.1447
中图分类号：
学科分类号：
摘要：
We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula for its precision exaggerates beyond Fisher's information limit, and weighted least-squares may do worse, whereas maximum-likelihood fitting is practically optimal. © 2010 Nature America, Inc. All rights reserved.
引用
收藏
页码：377 / 381
页数：4
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