Optimized localization analysis for single-molecule tracking and super-resolution microscopy

被引：2
|
作者：
Mortensen, Kim I. [1 ]
Churchman, L. Stirling [2 ,3 ]
Spudich, James A. [2 ]
Flyvbjerg, Henrik [1 ]
机构：
[1] Tech Univ Denmark, Dept Micro & Nanotechnol, Kongens Lyngby, Denmark
[2] Stanford Univ, Dept Biochem, Sch Med, Stanford, CA 94305 USA
[3] Stanford Univ, Dept Phys, Sch Med, Stanford, CA 94305 USA
来源：
NATURE METHODS | 2010年 / 7卷 / 05期
基金：
美国国家卫生研究院;
关键词：
HAND-OVER-HAND; MYOSIN-V; ORIENTATION; ACCURACY;
D O I：
10.1038/NMETH.1447
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula for its precision exaggerates beyond Fisher's information limit, and weighted least-squares may do worse, whereas maximum-likelihood fitting is practically optimal.
引用
收藏
页码：377 / U59
页数：7
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