Imaging theory and resolution improvement of two-photon confocal microscopy

被引：0
|
作者：
Tang Zhilie
Yang Chuping
Pei Hongjin
Liang Ruisheng
Liu Songhao
机构：
[1] South China Normal University,Department of Physics
[2] South China Agricultural University,College of Sciences
来源：
Science in China Series A: Mathematics | 2002年 / 45卷
关键词：
two-photon; confocal microscopy; resolution;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The nonlinear effect of two-photon excitation on the imaging property of two-photon confocal microscopy has been analyzed by the two-photon fluorescence intensity transfer function derived in this paper. The two-photon fluorescence intensity transfer function in a confocal microscopy is given. Furthermore the three-dimensional point spread function (3D-PSF) and the three-dimensional optical transfer function (3D-OTF) of two-photon confocal microscopy are derived based on the nonlinear effect of two-photon excitation. The imaging property of two-photon confocal microscopy is discussed in detail based on 3D-OTF. Finally the spatial resolution limit of two-photon confocal microscopy is discussed according to the uncertainty principle.
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收藏
页码：1468 / 1478
页数：10
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