Study on the Spatial Architecture of p53, MDM2, and p14ARF Containing Complexes

被引：0
|
作者：
Andrej Savchenko
Mariya Yurchenko
Boris Snopok
Elena Kashuba
机构：
[1] NASU,V. Lashkaryov Institute of Semiconductor Physics
[2] NASU,R. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
[3] Karolinska Institute,Department of Microbiology, Tumor, and Cell Biology (MTC)
[4] Karolinska Institute,IRIS (Center for Integrative Recognition in the Immune System)
来源：
Molecular Biotechnology | 2009年 / 41卷
关键词：
Supramolecular protein complexes; SPR; Spatial organization; Ternary complex; Co-localization;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We have developed a surface plasmon resonance (SPR)-based immunocapture approach to study multimeric protein–protein complexes. A composition and spatial architecture of protein complexes that contained GST-tagged p53, p14ARF, and MDM2 was examined by the developed approach. Obtained results verified that the p53 protein possesses two binding sites for MDM2. Ternary complexes containing p14ARF, MDM2, and p53 proteins could only be formed when MDM2 protein functions as a bridging molecule. That was confirmed by immunoprecipitation and immunostaining.
引用
收藏
相关论文
共 50 条
  • [41] Impact of MDM2, TP53 and P14ARF Polymorphisms on Endometrial Cancer Risk and Onset
    Wujcicka, Wioletta
    Zajac, Agnieszka
    Stachowiak, Grzegorz
    IN VIVO, 2019, 33 (03): : 917 - 924
  • [42] Cooperativity of p19ARF, Mdm2, and p53 in murine tumorigenesis
    Lynette Moore
    Sundaresan Venkatachalam
    Hannes Vogel
    Julie C Watt
    Chao-Ling Wu
    Heather Steinman
    Stephen N Jones
    Lawrence A Donehower
    Oncogene, 2003, 22 : 7831 - 7837
  • [43] Control of p53 ubiquitination and nuclear export by MDM2 and ARF
    Zhang, YP
    Xiong, Y
    CELL GROWTH & DIFFERENTIATION, 2001, 12 (04): : 175 - 186
  • [44] P53 escape from P19ARF-trapped MDM2
    Mariani, S
    NATURE MEDICINE, 1999, 5 (05) : 490 - 490
  • [45] Cooperativity of p19ARF, Mdm2, and p53 in murine tumorigenesis
    Moore, L
    Venkatachalam, S
    Vogel, H
    Watt, JC
    Wu, CL
    Steinman, H
    Jones, SN
    Donehower, LA
    ONCOGENE, 2003, 22 (49) : 7831 - 7837
  • [46] P53 escape from P19ARF-trapped MDM2
    Sara Mariani
    Nature Medicine, 1999, 5 : 490 - 490
  • [47] Genetic Variation in MDM2 and p14ARF and Susceptibility to Salivary Gland Carcinoma
    Jin, Lei
    Xu, Li
    Song, Xicheng
    Wei, Qingyi
    Sturgis, Erich M.
    Li, Guojun
    PLOS ONE, 2012, 7 (11):
  • [48] Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2
    Clark, PA
    Llanos, S
    Peters, G
    ONCOGENE, 2002, 21 (29) : 4498 - 4507
  • [49] p14ARF links the tumour suppressors RB and p53
    Bates, S
    Phillips, AC
    Clark, PA
    Stott, F
    Peters, G
    Ludwig, RL
    Vousden, KH
    NATURE, 1998, 395 (6698) : 124 - 125
  • [50] MDM2 and BCL-2: to p53 or not to p53?
    Wang, Eunice S.
    BLOOD, 2023, 141 (11) : 1237 - 1238
12345