Study on the Spatial Architecture of p53, MDM2, and p14ARF Containing Complexes

被引：0
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作者：
Andrej Savchenko
Mariya Yurchenko
Boris Snopok
Elena Kashuba
机构：
[1] NASU,V. Lashkaryov Institute of Semiconductor Physics
[2] NASU,R. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology
[3] Karolinska Institute,Department of Microbiology, Tumor, and Cell Biology (MTC)
[4] Karolinska Institute,IRIS (Center for Integrative Recognition in the Immune System)
来源：
Molecular Biotechnology | 2009年 / 41卷
关键词：
Supramolecular protein complexes; SPR; Spatial organization; Ternary complex; Co-localization;
D O I：
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学科分类号：
摘要：
We have developed a surface plasmon resonance (SPR)-based immunocapture approach to study multimeric protein–protein complexes. A composition and spatial architecture of protein complexes that contained GST-tagged p53, p14ARF, and MDM2 was examined by the developed approach. Obtained results verified that the p53 protein possesses two binding sites for MDM2. Ternary complexes containing p14ARF, MDM2, and p53 proteins could only be formed when MDM2 protein functions as a bridging molecule. That was confirmed by immunoprecipitation and immunostaining.
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