Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control

被引：0
|
作者：
Xing-Long Xiao
Ya-Qing He
Yi-Gang Yu
Hong Yang
Gu Chen
Hui-Fang Li
Jing-Wei Zhang
Dong-Mei Liu
Xiao-Feng Li
Xiao-Quan Yang
Hui Wu
机构：
[1] South China University of Technology,Department of Food, Institution of Food safety, College of Light Industry and Food
[2] Shenzhen Taitai Genomics,undefined
[3] Inc.,undefined
[4] Shenzhen Center for Disease Control and Prevention,undefined
来源：
Archives of Virology | 2009年 / 154卷
关键词：
Throat Swab; Rectal Swab; Internal Amplification Control; Throat Swab Specimen; ABI7500 Sequence Detector System;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.
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页码：121 / 125
页数：4
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