Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control

被引：0

作者：

Xing-Long Xiao

Ya-Qing He

Yi-Gang Yu

Hong Yang

Gu Chen

Hui-Fang Li

Jing-Wei Zhang

Dong-Mei Liu

Xiao-Feng Li

Xiao-Quan Yang

Hui Wu

机构：

[1] South China University of Technology,Department of Food, Institution of Food safety, College of Light Industry and Food

[2] Shenzhen Taitai Genomics,undefined

[3] Inc.,undefined

[4] Shenzhen Center for Disease Control and Prevention,undefined

来源：

Archives of Virology | 2009年 / 154卷

关键词：

Throat Swab; Rectal Swab; Internal Amplification Control; Throat Swab Specimen; ABI7500 Sequence Detector System;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.

引用

页码：121 / 125

页数：4

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