Protein detection: Optimization of Western blotting signals with chemiluminescence

被引:0
|
作者
Mabuchi M. [1 ]
Gutierrez S. [1 ]
Nadler T. [1 ]
Sibani S. [1 ]
Chandreyee D.A.S. [1 ]
机构
[1] Millipore Corporation, Billerica, MA
关键词
D O I
10.1007/s12268-011-0012-8
中图分类号
学科分类号
摘要
Using Western blotting to detect proteins in a cell lysáte is a powerful, complementary approach to immunocytochemistry. Successful Western blotting is dependent on the optimization of multiple variables. In this study, we focused on optimization of chemiluminescence detection.
引用
收藏
页码:62 / 63
页数:1
相关论文
共 50 条
  • [41] Stain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blotting
    Rivero-Gutierrez, B.
    Anzola, A.
    Martinez-Augustin, O.
    de Medina, F. Sanchez
    ANALYTICAL BIOCHEMISTRY, 2014, 467 : 1 - 3
  • [42] Application of Western-blotting technique with chemiluminescence imaging to the study of haptoglobin type and haptoglobin complexes
    Ouyang, J.
    Delanghe, J.
    Baeyens, W.R.G.
    Langlois, M.
    Biomedical Chromatography, 1999, 13 (02): : 148 - 150
  • [43] Glyco-western blotting: Biotinylated dermatan sulfate as a probe for the detection of dermatan sulfate binding proteins using western blotting
    Saito, A
    Munakata, H
    Satoh, K
    CONNECTIVE TISSUE RESEARCH, 2002, 43 (01) : 1 - 7
  • [44] Protein purification and analysis: next generation Western blotting techniques
    Mishra, Manish
    Tiwari, Shuchita
    Gomes, Aldrin V.
    EXPERT REVIEW OF PROTEOMICS, 2017, 14 (11) : 1037 - 1053
  • [45] FELINE CALICIVIRUS PROTEIN-SYNTHESIS INVESTIGATED BY WESTERN BLOTTING
    CARTER, MJ
    ARCHIVES OF VIROLOGY, 1989, 108 (1-2) : 69 - 79
  • [46] Western blotting with diaminobenzidine detection for the diagnosis of congenital disorders of glycosylation
    Artuch, R
    Ferrer, I
    Pineda, J
    Moreno, J
    Busquets, C
    Briones, P
    Vilaseca, MA
    JOURNAL OF NEUROSCIENCE METHODS, 2003, 125 (1-2) : 167 - 171
  • [47] A LIQUID GELATIN BLOCKING REAGENT FOR WESTERN BLOTTING WITH CHEMILUMINESCENT DETECTION
    LEE, SL
    STEVENS, J
    WANG, WW
    LANZILLO, JJ
    BIOTECHNIQUES, 1994, 17 (01) : 60 - 62
  • [48] LIGAND WESTERN BLOTTING FOR SPECIFIC DETECTION OF ACTIVE FORMS OF PROTEASES
    KIDO, T
    YOOK, HY
    UEDA, K
    CLINICA CHIMICA ACTA, 1995, 237 (1-2) : 31 - 41
  • [49] Western blotting analysis of the Plum pox virus capsid protein
    Kollerova, E.
    Glasa, M.
    Subr, Z. W.
    JOURNAL OF PLANT PATHOLOGY, 2008, 90 (01) : 19 - 22
  • [50] Use of west-western blotting for detection of adenovirus receptors
    Tarassishin, LA
    Russell, WC
    BIOCHEMISTRY-MOSCOW, 1997, 62 (01) : 38 - 40