Protein detection: Optimization of Western blotting signals with chemiluminescence

被引：0
|
作者：
Mabuchi M. [1 ]
Gutierrez S. [1 ]
Nadler T. [1 ]
Sibani S. [1 ]
Chandreyee D.A.S. [1 ]
机构：
[1] Millipore Corporation, Billerica, MA
来源：
BIOspektrum | 2011年 / 17卷 / 1期
关键词：
D O I：
10.1007/s12268-011-0012-8
中图分类号：
学科分类号：
摘要：
Using Western blotting to detect proteins in a cell lysáte is a powerful, complementary approach to immunocytochemistry. Successful Western blotting is dependent on the optimization of multiple variables. In this study, we focused on optimization of chemiluminescence detection.
引用
收藏
页码：62 / 63
页数：1
相关论文
共 50 条
  • [31] Emerging techniques of western blotting for purification and analysis of protein
    Singh, Krishna Kumar
    Gupta, Anshika
    Bharti, Charu
    Sharma, Himanchal
    FUTURE JOURNAL OF PHARMACEUTICAL SCIENCES, 2021, 7 (01)
  • [32] Measuring protein levels in planarians using western blotting
    Ziman, Benjamin
    Oviedo, Nestor J.
    STAR PROTOCOLS, 2021, 2 (01):
  • [33] Western Blotting
    Burnette, W. Neal
    CLINICAL CHEMISTRY, 2011, 57 (01) : 132 - 133
  • [34] Western blotting
    Kurien, BT
    Scofield, RH
    METHODS, 2006, 38 (04) : 283 - 293
  • [35] Detection of Zebrafish Retinal Proteins by Infrared Western Blotting
    Zang, Jingjing
    Neuhauss, Stephan C. F.
    BIO-PROTOCOL, 2023, 13 (04):
  • [36] ENHANCED CHEMILUMINESCENCE ASSAY FOR PEROXIDASE : APPLICATION IN IMMUNOASSAY, PROTEIN BLOTTING AND DNA HYBRIDIZATION
    Kricka, Larry J.
    Ji, Xiaoying
    ANALYTICAL SCIENCES, 1991, 7 : 1501 - 1506
  • [37] Photographic detection of fluorescent-labelled oligodeoxynucleotide in the blotting format by peroxyoxalate chemiluminescence
    Kuroda, N
    Hosoki, S
    Nakashima, K
    Akiyama, S
    Givens, RS
    JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE, 1998, 13 (02): : 101 - 105
  • [38] Photographic Detection of Fluorescent-Labelled Oligodeoxynucleotide in the Blotting Format by Peroxyoxalate Chemiluminescence
    Kuroda, Naotaka
    Hosoki, Sayuri
    Nakashima, Kenichiro
    Akiyama, Shuzo
    Givens, Richard S.
    Luminescence, 13 (02): : 101 - 105
  • [39] Application of Western-blotting technique with chemiluminescence imaging to the study of haptoglobin type and haptoglobin complexes
    Ouyang, J
    Delanghe, J
    Baeyens, WRG
    Langlois, M
    ANALYTICA CHIMICA ACTA, 1998, 362 (2-3) : 113 - 120
  • [40] p53 protein detection by the Western blotting technique in normal and neoplastic specimens of human endometrium
    Brys, M
    Semczuk, A
    Wójcik, M
    Krajewska, WM
    Jakowicki, JA
    CANCER LETTERS, 2000, 148 (02) : 197 - 205
12345