Thiopurine S-methyltransferase activity in human erythrocytes: a new HPLC method using 6-thioguanine as substrate

被引：0
|
作者：
T. Kröplin
N. Weyer
S. Gutsche
H. Iven
机构：
[1] Department of Pharmacology,
[2] Lübeck Medical University,undefined
[3] Ratzeburger Allee 160,undefined
[4] D-23538 Lübeck,undefined
[5] Germany Tel. +49-451-500 2682; Fax +49-451-500 2686,undefined
来源：
European Journal of Clinical Pharmacology | 1998年 / 54卷
关键词：
Key words Thiopurine methyltransferase; Polymorphism;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Objectives: To develop a non-radioactive assay to measure thiopurine S-methyltransferase (TPMT) activity. The assay was used to study the distribution of TPMT activity in a healthy German population.
引用
收藏
页码：265 / 271
页数：6
相关论文
共 50 条
  • [21] Why measure thiopurine methyltransferase activity? Direct administration of 6-thioguanine might be the alternative for 6-mercaptopurine or azathioprine
    de Jong, D
    Mulder, CJJ
    van Sorge, AA
    GUT, 2001, 49 (06) : 874 - 874
  • [22] Methylation of 6-mercaptopurine and 6-thioguanine by thiopurine S-methyltransferase A comparison of activity in red blood cell samples of 199 blood donorsA comparison of activity in red blood cell samples of 199 blood donors
    T. Kröplin
    H. Iven
    European Journal of Clinical Pharmacology, 2000, 56 : 343 - 345
  • [23] Interference free and simplyfied liquid chromatography-based determination of thiopurine S-methyltransferase activity in erythrocytes
    Khalil, MN
    Erb, N
    Khalil, PN
    Escherich, G
    Janka-Schaub, GE
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 821 (01): : 105 - 111
  • [24] Possibilities for therapeutic drug monitoring of azathioprine: 6-Thioguanine nucleotide concentrations and thiopurine methyltransferase activity in red blood cells
    Bergan, S
    Rugstad, HE
    Klemetsdal, B
    Giverhaug, T
    Bentdal, O
    Sodal, G
    Hartmann, A
    Aarbakke, J
    Stokke, O
    THERAPEUTIC DRUG MONITORING, 1997, 19 (03) : 318 - 326
  • [25] High Thiopurine Methyltransferase Activity Does Not Affect Concentration of Azathioprine Metabolite 6-Thioguanine Nucleotide in Inflammatory Bowel Disease
    Jonason, David
    Sievers, Tyson
    Abraham, James M.
    Vaughn, Byron
    AMERICAN JOURNAL OF GASTROENTEROLOGY, 2019, 114 : S461 - S462
  • [26] A single point mutation leading to loss of catalytic activity in human thiopurine S-methyltransferase
    Proceedings of the National Academy of Sciences of the United States of America, 92 (04):
  • [27] Thiopurine methyltransferase activity combined with 6-thioguanine metabolite levels predicts clinical response to thiopurines in patients with inflammatory bowel disease
    Kwan, L. Y.
    Devlin, S. M.
    Mirocha, J. M.
    Papadakis, K. A.
    DIGESTIVE AND LIVER DISEASE, 2008, 40 (06) : 425 - 432
  • [28] Molecular dynamics simulation of a human thiopurine S-methyltransferase complexed with 6-mercaptopurine model
    Mokmak, Wanwimon
    Tongsima, Sissades
    Jenwitheesuk, Ekachai
    BIOINFORMATION, 2009, 4 (02) : 59 - 62
  • [29] A SINGLE-POINT MUTATION LEADING TO LOSS OF CATALYTIC ACTIVITY IN HUMAN THIOPURINE S-METHYLTRANSFERASE
    KRYNETSKI, EY
    SCHUETZ, JD
    GALPIN, AJ
    PUI, CH
    RELLING, MV
    EVANS, WE
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (04) : 949 - 953
  • [30] Whole-blood thiopurine S-methyltransferase activity with genotype concordance:: a new, simplified phenotyping assay
    Ford, Loretta
    Graham, Valerie
    Berg, Jonathan
    ANNALS OF CLINICAL BIOCHEMISTRY, 2006, 43 : 354 - 360
12345