Activity of promoters of carnation etched ring virus and dahlia mosaic virus in tobacco protoplasts and transgenic plants

被引：0
|
作者：
B. R. Kuluev
A. V. Knyazev
A. V. Chemeris
机构：
[1] Russian Academy of Sciences,Institute of Biochemistry and Genetics, Ufa Research Center
来源：
Russian Journal of Plant Physiology | 2008年 / 55卷
关键词：
caulimoviruses; 35S promoter; carnation etched ring virus; dahlia mosaic virus; GUS; GFP; X-Gluc; 4-MUG;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Promoters of carnation etched ring virus (CERV) and dahlia mosaic virus (DMV) were cloned into binary vectors pCambia 1304, pCambia 1281Z, and pCambia 1291Z with reporter GFP and GUS genes. Activities of these promoters in tobacco protoplasts and transgenic plants were determined using these constructs. Histochemical GUS analysis demonstrated the absence of tissue-specificity in transgenic plants transformed with these promoters. The quantitative analysis of these promoter activities in transgenic tobacco plants, using 4-methylumbelliferone as a substrate, showed that 35S CaMV, CERV, and DMV promoters displayed approximately similar activities in transgenic tobacco plants.
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页码：687 / 693
页数：6
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