Activity of promoters of carnation etched ring virus and dahlia mosaic virus in tobacco protoplasts and transgenic plants

被引：0
|
作者：
B. R. Kuluev
A. V. Knyazev
A. V. Chemeris
机构：
[1] Russian Academy of Sciences,Institute of Biochemistry and Genetics, Ufa Research Center
来源：
Russian Journal of Plant Physiology | 2008年 / 55卷
关键词：
caulimoviruses; 35S promoter; carnation etched ring virus; dahlia mosaic virus; GUS; GFP; X-Gluc; 4-MUG;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Promoters of carnation etched ring virus (CERV) and dahlia mosaic virus (DMV) were cloned into binary vectors pCambia 1304, pCambia 1281Z, and pCambia 1291Z with reporter GFP and GUS genes. Activities of these promoters in tobacco protoplasts and transgenic plants were determined using these constructs. Histochemical GUS analysis demonstrated the absence of tissue-specificity in transgenic plants transformed with these promoters. The quantitative analysis of these promoter activities in transgenic tobacco plants, using 4-methylumbelliferone as a substrate, showed that 35S CaMV, CERV, and DMV promoters displayed approximately similar activities in transgenic tobacco plants.
引用
收藏
页码：687 / 693
页数：6
相关论文
共 50 条
  • [21] Phosphorylation of the Movement Protein of Cucumber Mosaic Virus in Transgenic Tobacco Plants
    Yasuhiko Matsushita
    Kuniaki Yoshioka
    Takuma Shigyo
    Hideki Takahashi
    Hiroshi Nyunoya
    Virus Genes, 2002, 24 : 231 - 234
  • [22] ULTRASTRUCTURE OF INFECTION OF TOBACCO MESOPHYLL PROTOPLASTS BY TOBACCO MOSAIC VIRUS
    OTSUKI, Y
    TAKEBE, I
    MATSUI, C
    HONDA, Y
    VIROLOGY, 1972, 49 (01) : 188 - &
  • [23] RETENTION AND DISSOCIATION OF TOBACCO MOSAIC-VIRUS BY TOBACCO PROTOPLASTS
    WYATT, SD
    SHAW, JG
    VIROLOGY, 1975, 63 (02) : 459 - 465
  • [24] BIOSYNTHESIS OF TOBACCO MOSAIC-VIRUS RNA IN TOBACCO PROTOPLASTS
    SINDELAR, L
    SINDELAROVA, M
    BIOLOGIA PLANTARUM, 1995, 37 (02) : 285 - 290
  • [25] INFECTION OF TOBACCO MESOPHYLL PROTOPLASTS WITH TOBACCO MOSAIC-VIRUS
    COUTTS, RHA
    COCKING, EC
    KASSANIS, B
    JOURNAL OF GENERAL VIROLOGY, 1972, 17 (DEC): : 289 - 294
  • [26] EXPRESSION OF ALFALFA MOSAIC-VIRUS AND TOBACCO RATTLE VIRUS COAT PROTEIN GENES IN TRANSGENIC TOBACCO PLANTS
    VANDUN, CMP
    BOL, JF
    VANVLOTENDOTING, L
    VIROLOGY, 1987, 159 (02) : 299 - 305
  • [27] VIRUS-RESISTANCE OF TRANSGENIC TOBACCO PLANTS EXPRESSING TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE
    TIAN, YC
    QIN, XF
    WANG, GL
    YANG, MZ
    FANG, RX
    XU, BY
    MANG, KQ
    SCIENCE IN CHINA SERIES B-CHEMISTRY, 1991, 34 (11): : 1329 - 1340
  • [28] INFECTION OF COWPEA MESOPHYLL PROTOPLASTS BY TOBACCO MOSAIC-VIRUS AN TOBACCO NECROSIS VIRUS
    WIERINGABRANTS, DH
    TIMMER, FA
    ROUWELER, MHC
    NETHERLANDS JOURNAL OF PLANT PATHOLOGY, 1978, 84 (06): : 239 - 240
  • [29] Synthetic Promoters from Strawberry Vein Banding Virus (SVBV) and Dahlia Mosaic Virus (DaMV)
    Badrinath Khadanga
    Jeky Chanwala
    I. Sriram Sandeep
    Nrisingha Dey
    Molecular Biotechnology, 2021, 63 : 792 - 806
  • [30] Synthetic Promoters from Strawberry Vein Banding Virus (SVBV) and Dahlia Mosaic Virus (DaMV)
    Khadanga, Badrinath
    Chanwala, Jeky
    Sandeep, I. Sriram
    Dey, Nrisingha
    MOLECULAR BIOTECHNOLOGY, 2021, 63 (09) : 792 - 806
12345