Backbone resonance assignments for the SET domain of human methyltransferase NSD3 in complex with its cofactor

被引：0
|
作者：
Yan Li
Hui Qi Ng
Anna Ngo
Shuang Liu
Yih Wan Tan
Perlyn Zekui Kwek
Alvin W. Hung
Joma Joy
Jeffrey Hill
Thomas H. Keller
CongBao Kang
机构：
[1] Agency for Science,Experimental Therapeutics Centre
[2] Technology and Research,undefined
来源：
Biomolecular NMR Assignments | 2017年 / 11卷
关键词：
NSD3; Methyltransferase; SET domain; Backbone assignment;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
NSD3 is a histone H3 methyltransferase that plays an important role in chromatin biology. A construct containing the methyltransferase domain encompassing residues Q1049-K1299 of human NSD3 was obtained and biochemical activity was demonstrated using histone as a substrate. Here we report the backbone HN, N, Cα, C′, and side chain Cβ assignments of the construct in complex with S-adenosyl-l-methionine (SAM). Based on these assignments, secondary structures of NSD3/SAM complex in solution were determined.
引用
收藏
页码：225 / 229
页数：4
相关论文
共 50 条
  • [41] Letter to the editor:: 1H, 13C and 15N resonance assignments of a viral SET domain histone lysine methyltransferase
    Manzur, K
    Farooq, A
    Zeng, L
    Zhou, MM
    JOURNAL OF BIOMOLECULAR NMR, 2003, 26 (03) : 279 - 280
  • [42] Polypeptide backbone, Cβ and methyl group resonance assignments of the 24 kDa plectin repeat domain 6 from human protein plectin
    Pulavarti, Surya V. S. R. K.
    Eletsky, Alexander
    Huang, Yuanpeng J.
    Acton, Thomas B.
    Xiao, Rong
    Everett, John K.
    Montelione, Gaetano T.
    Szyperski, Thomas
    BIOMOLECULAR NMR ASSIGNMENTS, 2015, 9 (01) : 135 - 138
  • [43] Letter to the Editor:: Backbone 1H, 15N, and 13C resonance assignments of the repeated domain of human βig-h3 protein
    Park, S
    Ahn, HC
    Kim, JE
    Kim, IS
    Lee, BJ
    JOURNAL OF BIOMOLECULAR NMR, 2002, 24 (04) : 367 - 368
  • [44] Backbone NMR assignments of the C-terminal domain of the human prion protein and its disease‐associated T183A variant
    Máximo Sanz-Hernández
    Alfonso De Simone
    Biomolecular NMR Assignments, 2021, 15 : 193 - 196
  • [45] Letter to the Editor: Backbone 1H, 15N, and 13C resonance assignments of the repeated domain of human βig-h3 protein
    Sangho Park
    Hee-Chul Ahn
    Jung-Eun Kim
    In-San Kim
    Bong-Jin Lee
    Journal of Biomolecular NMR, 2002, 24 : 367 - 368
  • [46] Backbone NMR resonance assignments of the nucleotide binding domain of the ABC multidrug transporter LmrA from Lactococcus lactis in its ADP-bound state
    Hellmich, Ute A.
    Duchardt-Ferner, Elke
    Glaubitz, Clemens
    Woehnert, Jens
    BIOMOLECULAR NMR ASSIGNMENTS, 2012, 6 (01) : 69 - 73
  • [47] Backbone NMR resonance assignments of the nucleotide binding domain of the ABC multidrug transporter LmrA from Lactococcus lactis in its ADP-bound state
    Ute A. Hellmich
    Elke Duchardt-Ferner
    Clemens Glaubitz
    Jens Wöhnert
    Biomolecular NMR Assignments, 2012, 6 : 69 - 73
  • [48] 1H, 15N and 13C backbone resonance assignments of the acidic domain of the human MDM2 protein
    Song, Qinyan
    Liu, Xiang-Qin
    Rainey, Jan K.
    BIOMOLECULAR NMR ASSIGNMENTS, 2023, 17 (01) : 9 - 16
  • [49] 1H, 15N and 13C backbone resonance assignments of the acidic domain of the human MDM2 protein
    Qinyan Song
    Xiang-Qin Liu
    Jan K. Rainey
    Biomolecular NMR Assignments, 2023, 17 : 9 - 16
  • [50] Backbone NMR assignments of the C-terminal domain of the human prion protein and its disease-associated T183A variant
    Sanz-Hernandez, Maximo
    De Simone, Alfonso
    BIOMOLECULAR NMR ASSIGNMENTS, 2021, 15 (01) : 193 - 196
12345