Super-resolution microscopy with DNA-PAINT

被引：0
|
作者：
Joerg Schnitzbauer
Maximilian T Strauss
Thomas Schlichthaerle
Florian Schueder
Ralf Jungmann
机构：
[1] Ludwig Maximilian University,Department of Physics and Center for Nanoscience
[2] Max Planck Institute of Biochemistry,undefined
来源：
Nature Protocols | 2017年 / 12卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In DNA-PAINT, transient binding of dye-labeled oligonucleotides to their target strands creates the ‘blinking’ required for stochastic nanoscopy. This protocol describes how to apply DNA-PAINT, from sample preparation to data processing.
引用
收藏
页码：1198 / 1228
页数：30
相关论文
共 50 条
  • [41] DNA Origami as a Nanoscopic Ruler for Super-Resolution Microscopy
    Steinhauer, Christian
    Jungmann, Ralf
    Sobey, Thomas L.
    Simmel, Friedrich C.
    Tinnefeld, Philip
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (47) : 8870 - 8873
  • [42] Quantitative Super-Resolution Microscopy using DNA Origami
    Zanacchi, Francesca Cella
    Magrassi, Raffaella
    Diaspro, Alberto
    Manzo, Carlo
    Derr, Nathan D.
    Lakadamyali, Melike
    BIOPHYSICAL JOURNAL, 2018, 114 (03) : 187A - 187A
  • [43] Multiplexed Lectin-PAINT super-resolution microscopy enables cell glycotyping
    Tholen, Marrit M. E.
    Riera, Roger
    Izquierdo-Lozano, Cristina
    Albertazzi, Lorenzo
    COMMUNICATIONS BIOLOGY, 2025, 8 (01)
  • [44] Quantitative comparison of spinning disk geometries for PAINT based super-resolution microscopy
    Sirinakis, George
    Allgeyer, Edward S.
    Cheng, Jinmei
    St Johnston, Daniel
    BIOMEDICAL OPTICS EXPRESS, 2022, 13 (07): : 3773 - 3785
  • [45] Site-Specific Labeling of Affimers for DNA-PAINT Microscopy
    Schlichthaerle, Thomas
    Eklund, Alexandra S.
    Schueder, Florian
    Strauss, Maximilian T.
    Tiede, Christian
    Curd, Alistair
    Ries, Jonas
    Peckham, Michelle
    Tomlinson, Darren C.
    Jungmann, Ralf
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 57 (34) : 11060 - 11063
  • [46] Recent progress on super-resolution imaging and correlative super-resolution microscopy
    Lin Dan-Ying
    Qu Jun-Le
    ACTA PHYSICA SINICA, 2017, 66 (14)
  • [47] Super-resolution microscopy of mitochondria
    Jakobs, Stefan
    Wurm, Christian A.
    CURRENT OPINION IN CHEMICAL BIOLOGY, 2014, 20 : 9 - 15
  • [48] Gentler super-resolution microscopy
    Strack, Rita
    NATURE METHODS, 2018, 15 (10) : 764 - 764
  • [49] Introduction to super-resolution microscopy
    Yamanaka, Masahito
    Smith, Nicholas I.
    Fujita, Katsumasa
    MICROSCOPY, 2014, 63 (03) : 177 - 192
  • [50] Super-resolution microscopy demystified
    Schermelleh, Lothar
    Ferrand, Alexia
    Huser, Thomas
    Eggeling, Christian
    Sauer, Markus
    Biehlmaier, Oliver
    Drummen, Gregor P. C.
    NATURE CELL BIOLOGY, 2019, 21 (01) : 72 - 84
12345