Super-resolution microscopy with DNA-PAINT

被引：0
|
作者：
Joerg Schnitzbauer
Maximilian T Strauss
Thomas Schlichthaerle
Florian Schueder
Ralf Jungmann
机构：
[1] Ludwig Maximilian University,Department of Physics and Center for Nanoscience
[2] Max Planck Institute of Biochemistry,undefined
来源：
Nature Protocols | 2017年 / 12卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In DNA-PAINT, transient binding of dye-labeled oligonucleotides to their target strands creates the ‘blinking’ required for stochastic nanoscopy. This protocol describes how to apply DNA-PAINT, from sample preparation to data processing.
引用
收藏
页码：1198 / 1228
页数：30
相关论文
共 50 条
  • [21] Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT
    Jungmann R.
    Avendaño M.S.
    Woehrstein J.B.
    Dai M.
    Shih W.M.
    Yin P.
    Nature Methods, 2014, 11 (3) : 313 - 318
  • [22] Super-resolution imaging and estimation of protein copy numbers at single synapses with DNA-PAINT
    Boeger, Carolin
    Hafner, Anne-Sophie
    Schlichthaerle, Thomas
    Strauss, Maximilian T.
    Malkusch, Sebastian
    Endesfelder, Ulrike
    Jungmann, Ralf
    Schuman, Erin M.
    Heilemann, Mike
    NEUROPHOTONICS, 2019, 6 (03)
  • [23] 124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics
    Wade, Orsolya K.
    Woehrstein, Johannes B.
    Nickels, Philipp C.
    Strauss, Sebastian
    Stehr, Florian
    Stein, Johannes
    Schueder, Florian
    Strauss, Maximilian T.
    Ganji, Mahipal
    Schnitzbauer, Joerg
    Grabmayr, Heinrich
    Yin, Peng
    Schwille, Petra
    Jungmann, Ralf
    NANO LETTERS, 2019, 19 (04) : 2641 - 2646
  • [24] DNA-PAINT super-resolution imaging data of surface exposed active sites on particles
    Delcanale, Pietro
    Albertazzi, Lorenzo
    DATA IN BRIEF, 2020, 30
  • [25] Beyond DNA: new probes for PAINT super-resolution microscopy
    Tholen, Marrit M. E.
    Tas, Roderick P.
    Wang, Yuyang
    Albertazzi, Lorenzo
    CHEMICAL COMMUNICATIONS, 2023, 59 (54) : 8332 - 8342
  • [26] Correlating DNA-PAINT and single-molecule FRET for multiplexed super-resolution imaging
    Deussner-Helfmann, Nina S.
    Auer, Alexander
    Strauss, Maximilian T.
    Donlin-Asp, Paul
    Malkusch, Sebastian
    Dietz, Marina S.
    Barth, Hans-Dieter
    Schuman, Erin
    Jungmann, Ralf
    Heilemann, Mike
    SINGLE MOLECULE SPECTROSCOPY AND SUPERRESOLUTION IMAGING XIII, 2020, 11246
  • [27] Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy
    Zahringer, Jonas
    Cole, Fiona
    Bohlen, Johann
    Steiner, Florian
    Kaminska, Izabela
    Tinnefeld, Philip
    LIGHT-SCIENCE & APPLICATIONS, 2023, 12 (01)
  • [28] Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy
    Jonas Zähringer
    Fiona Cole
    Johann Bohlen
    Florian Steiner
    Izabela Kamińska
    Philip Tinnefeld
    Light: Science & Applications, 12
  • [29] 3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples
    Lin, Ruisheng
    Clowsley, Alexander H.
    Lutz, Tobias
    Baddeley, David
    Soeller, Christian
    METHODS, 2020, 174 : 56 - 71
  • [30] Nanobodies combined with DNA-PAINT super-resolution reveal a staggered titin nanoarchitecture in flight muscles
    Schueder, Florian
    Mangeol, Pierre
    Chan, Eunice HoYee
    Rees, Renate
    Schuenemann, Juergen
    Jungmann, Ralf
    Goerlich, Dirk
    Schnorrer, Frank
    ELIFE, 2023, 12
12345