Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of juncaceae

被引：0
|
作者：
Lenka Drábková
Jan Kirschner
Ĉestmír Vlĉek
机构：
[1] Academy of Sciences,Institute of Botany
[2] Academy of Sciences,Centre for Integrated Genomics and Institute of Molecular Genetics
来源：
Plant Molecular Biology Reporter | 2002年 / 20卷
关键词：
cpDNA; DNA extraction; herbarium specimens; Juncaceae; PCR amplification;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Seven DNA extraction protocols were used to obtain DNA from herbarium specimens ofJuncus andLuzula (Juncaceae) of various ages. DNA of historical samples is difficult to extract, and the extracts are seldom of good quality. The quality of DNA obtained was estimated by using a spectrophotometer to measure the A260/280 absorbance ratio. The total DNA yield was measured by a fluorometer. The results indicate the success of using both mixer mill grinding and a DNeasy Plant Kit. Another extraction protocol (grinding with mortar and pestle, using liquid nitrogen) yielded DNA from many samples. Modified CTAB extraction, with a lengthy precipitation, usually provided good amounts of DNA. Other protocols did not give satisfactory results.
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页码：161 / 175
页数：14
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