Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of juncaceae

被引：0

作者：

Lenka Drábková

Jan Kirschner

Ĉestmír Vlĉek

机构：

[1] Academy of Sciences,Institute of Botany

[2] Academy of Sciences,Centre for Integrated Genomics and Institute of Molecular Genetics

来源：

Plant Molecular Biology Reporter | 2002年 / 20卷

关键词：

cpDNA; DNA extraction; herbarium specimens; Juncaceae; PCR amplification;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Seven DNA extraction protocols were used to obtain DNA from herbarium specimens ofJuncus andLuzula (Juncaceae) of various ages. DNA of historical samples is difficult to extract, and the extracts are seldom of good quality. The quality of DNA obtained was estimated by using a spectrophotometer to measure the A260/280 absorbance ratio. The total DNA yield was measured by a fluorometer. The results indicate the success of using both mixer mill grinding and a DNeasy Plant Kit. Another extraction protocol (grinding with mortar and pestle, using liquid nitrogen) yielded DNA from many samples. Modified CTAB extraction, with a lengthy precipitation, usually provided good amounts of DNA. Other protocols did not give satisfactory results.

引用

页码：161 / 175

页数：14

共 50 条

[41] Comparison of DNA extraction protocols of fish fin and larva samples: modified salt (NaCl) extraction
Lopera-Barrero, Nelson M.
Povh, Jayme A.
Ribeiro, Ricardo P.
Gomes, Patricia C.
Jacometo, Carolina B.
Lopes, Tais da Silva
CIENCIA E INVESTIGACION AGRARIA, 2008, 35 (01): : 77 - 86
[42] Comparison of seven methods of DNA extraction from termitarium for functional metagenomic DNA library construction
Manjula, A.
Sathyavathi, S.
Gunasekaran, P.
Rajendhran, J.
JOURNAL OF SCIENTIFIC & INDUSTRIAL RESEARCH, 2011, 70 (11): : 945 - 951
[43] Comparison of seven RNA extraction methods on stool and shellfish samples prior to hepatitis A virus amplification
Arnal, C
Ferré-Aubineau, V
Besse, B
Mignotte, B
Schwartzbrod, L
Billaudel, S
JOURNAL OF VIROLOGICAL METHODS, 1999, 77 (01) : 17 - 26
[44] Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Leite, D. C. A.
Balieiro, F. C.
Pires, C. A.
Madari, B. E.
Rosado, A. S.
Coutinho, H. L. C.
Peixoto, R. S.
BRAZILIAN JOURNAL OF MICROBIOLOGY, 2014, 45 (01) : 175 - 183
[45] Comparison of DNA extraction protocols to detect Mycobacterium bovis in bovine tissue by PCR
Ikuta, Cassia Yumi
Ribeiro Oliveira, Daniella Carvalho
de Souza, Gisele Oliveira
de Souza Filho, Antonio Francisco
Hildebrand Grisi-Filho, Jose Henrique
Heinemann, Marcos Bryan
Ferreira Neto, Jose Soares
SEMINA-CIENCIAS AGRARIAS, 2016, 37 (05): : 3709 - 3718
[46] Evaluation of DNA extraction protocols from liquid-based cytology specimens for studying cervical microbiota
Shibata, Takeo
Nakagawa, Mayumi
Coleman, Hannah N.
Owens, Sarah M.
Greenfield, William W.
Sasagawa, Toshiyuki
Robeson, Michael S., II
PLOS ONE, 2021, 16 (08):
[47] Simple DNA extraction for museum beetle specimens to unlock genetic data from historical collections
Al-Dhafer, Hathal M.
Balaji, Raju
Abdel-Dayem, Mahmoud S.
Rasool, Iftekhar
Mohamed, Amr
Palanisamy, Senthilkumar
METHODSX, 2025, 14
[48] Development of DNA Extraction and PCR Amplification Protocols for Detection of Mycoplasma bovis Directly from Milk Samples
P. Cremonesi
C. Vimercati
G. Pisoni
G. Perez
A. Miranda Ribera
B. Castiglioni
M. Luzzana
G. Ruffo
P. Moroni
Veterinary Research Communications, 2007, 31 : 225 - 227
[49] Development of DNA extraction and PCR amplification Protocols for detection of Mycoplasma bovis directly from milk samples
Cremonesi, P.
Vimercati, C.
Pisoni, G.
Perez, G.
Ribera, A. Miranda
Castiglioni, B.
Luzzana, M.
Ruff, G.
Moroni, P.
VETERINARY RESEARCH COMMUNICATIONS, 2007, 31 (Suppl 1) : 225 - 227
[50] Enhanced DNA extraction and PCR amplification of mitochondrial genes from formalin-fixed museum specimens
Shedlock, AM
Haygood, MG
Pietsch, TW
Bentzen, P
BIOTECHNIQUES, 1997, 22 (03) : 394 - &

← 1 2 3 4 5 →