Extraction and amplification of nuclear DNA from herbarium specimens of emmer wheat: a method for assessing DNA preservation by maximum amplicon length recovery

被引：31
|
作者：
Lister, Diane L. [3 ]
Bower, Mim A. [3 ]
Howe, Christopher J. [2 ]
Jones, Martin K. [1 ]
机构：
[1] Univ Cambridge, Dept Archaeol, Cambridge CB2 3DZ, England
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[3] Univ Cambridge, McDonald Inst Archaeol Res, Cambridge CB2 3ER, England
来源：
TAXON | 2008年 / 57卷 / 01期
关键词：
ancient DNA; DNA preservation; herbarium specimens; HMW glutenin; PCR; Triticum turgidum subsp dicoccum;
D O I：
10.2307/25065966
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In this paper we present a simple and reliable method for the extraction of nuclear DNA from grains of herbarium specimens of emmer wheat, along with a method to determine the level of DNA preservation with a set of PCR primers based on the nuclear high-molecular-weight glutenin gene. The method gave PCR products of between 80 and 1,030 bp. PCR products from each set of amplification reactions were cloned and sequenced in order to confirm their identity. We have found excellent levels of DNA preservation in grain between 49 and 107 years old, consistently amplifying products of at least 350 bp.
引用
收藏
页码：254 / 258
页数：5
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