Extraction and amplification of nuclear DNA from herbarium specimens of emmer wheat: a method for assessing DNA preservation by maximum amplicon length recovery

被引：31
|
作者：
Lister, Diane L. [3 ]
Bower, Mim A. [3 ]
Howe, Christopher J. [2 ]
Jones, Martin K. [1 ]
机构：
[1] Univ Cambridge, Dept Archaeol, Cambridge CB2 3DZ, England
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[3] Univ Cambridge, McDonald Inst Archaeol Res, Cambridge CB2 3ER, England
来源：
TAXON | 2008年 / 57卷 / 01期
关键词：
ancient DNA; DNA preservation; herbarium specimens; HMW glutenin; PCR; Triticum turgidum subsp dicoccum;
D O I：
10.2307/25065966
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In this paper we present a simple and reliable method for the extraction of nuclear DNA from grains of herbarium specimens of emmer wheat, along with a method to determine the level of DNA preservation with a set of PCR primers based on the nuclear high-molecular-weight glutenin gene. The method gave PCR products of between 80 and 1,030 bp. PCR products from each set of amplification reactions were cloned and sequenced in order to confirm their identity. We have found excellent levels of DNA preservation in grain between 49 and 107 years old, consistently amplifying products of at least 350 bp.
引用
收藏
页码：254 / 258
页数：5
相关论文
共 50 条
  • [31] Enhanced DNA extraction and PCR amplification of mitochondrial genes from formalin-fixed museum specimens
    Shedlock, AM
    Haygood, MG
    Pietsch, TW
    Bentzen, P
    BIOTECHNIQUES, 1997, 22 (03) : 394 - &
  • [32] A DNA EXTRACTION METHOD THAT ALLOWS RELIABLE PCR AMPLIFICATION OF MLO DNA FROM DIFFICULT PLANT HOST SPECIES
    GIBB, K
    PADOVAN, A
    PCR-METHODS AND APPLICATIONS, 1994, 4 (01): : 56 - 58
  • [33] Extraction of single-copy nuclear DNA from forensic specimens with a variety of postmortem histories
    Evison, MP
    Smillie, DM
    Chamberlain, AT
    JOURNAL OF FORENSIC SCIENCES, 1997, 42 (06) : 1032 - 1038
  • [34] A simple method for inhibition free PCR amplification of target DNA directly from clinical specimens
    Senthilkumar Kamatchiammal
    Dhashinamoorthy Saravanakumar
    Nagalingeswaran Kumarasamy
    Sunithi Solomon
    Manjula Sritharan
    Venkataraman Sritharan
    Indian Journal of Clinical Biochemistry, 1997, 12 (1) : 78 - 80
  • [35] A novel and non-invasive method for DNA extraction from dry bee specimens
    Cilia, Giovanni
    Flaminio, Simone
    Quaranta, Marino
    SCIENTIFIC REPORTS, 2022, 12 (01)
  • [36] A novel and non-invasive method for DNA extraction from dry bee specimens
    Giovanni Cilia
    Simone Flaminio
    Marino Quaranta
    Scientific Reports, 12
  • [37] A rapid method for high throughput DNA extraction from plant material for PCR amplification
    Dilworth, E
    Frey, JE
    PLANT MOLECULAR BIOLOGY REPORTER, 2000, 18 (01) : 61 - 64
  • [38] A rapid method for high throughput DNA extraction from plant material for PCR amplification
    Eve Dilworth
    Juerg E. Frey
    Plant Molecular Biology Reporter, 2000, 18 : 61 - 64
  • [39] Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces
    Nechvatal, Jordan M.
    Ram, Jefftey L.
    Basson, Marc D.
    Namprachan, Phanramphoei
    Niec, Stephanie R.
    Badsha, Kawsar Z.
    Matherly, Larry H.
    Majumdar, Adhip P. N.
    Kato, Ikuko
    JOURNAL OF MICROBIOLOGICAL METHODS, 2008, 72 (02) : 124 - 132
  • [40] A MODIFIED METHOD OF DNA EXTRACTION FROM PERIPHERAL-BLOOD AND BONE-MARROW SPECIMENS
    HANSON, CA
    KERSEY, JH
    AMERICAN JOURNAL OF HEMATOLOGY, 1988, 28 (03) : 176 - 180
12345