Effects of amyloid-β peptide Aβ25–35 on glycolytic and antioxidant enzymes in erythrocytes of different ages

Amyloid-β peptide Aβ25–35 was shown to cause lysis of rat erythrocytes of different ages. The toxicity of Aβ25–35 positively correlated with both the erythrocyte age and the peptide concentration. The activity of glycolytic, antioxidant, and Na+/K+-ATPase enzymes decreased with erythrocyte aging in vivo. In vitro Aβ25–35 reduced the activity of hexokinase, phosphofructokinase, pyruvate kinase, glutathione peroxidase, and glutathione transferase and increased Na+/K+-ATPase activity in aged erythrocytes to a greater degree than in young cells.