Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli

被引：0
|
作者：
Paulo C. Bessa
A. J. Pedro
B. Klösch
A. Nobre
M. van Griensven
R. L. Reis
M. Casal
机构：
[1] University of Minho,3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering
[2] IBB – Institute for Biotechnology and Bioengineering,PT Government Associated Laboratory
[3] University of Minho,CBMA – Molecular and Environmental Biology Centre/Biology Department
[4] Ludwig Boltzmann Institute for Experimental and Clinical Traumatology,undefined
来源：
Biotechnology Letters | 2008年 / 30卷
关键词：
Bone morphogenetic protein-2; Recombinant protein purification; Stem cells; Tissue engineering;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity.
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页码：15 / 21
页数：6
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