Production and characterization of calcium phosphate cement incorporated with platelet concentrate

Calcium phosphate cements (CPCs) incorporated with platelet concentrate (PC) were prepared and characterized in an attempt to improve their bone regenerating performance. The characteristics of CPCs, such as paste injectability, initial setting time, and mechanical strength, were assessed. The generation of hydroxyapatite (HA) was observed with X-Ray diffraction (XRD) and scanning electron microscopy (SEM). The release of platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast growth factor (bFGF) was measured using enzyme linked immunosorbent assay (ELISA). The culture of Saos-2 cells was performed to assay the cell compatibility of CPCs. The paste injectability was enhanced but the cement setting was delayed by the incorporation of PC. The diametral tensile and compressive strengths were deteriorated by incorporated PC. The generation of HA was not affected by PC incorporation. The cumulative concentrations of released PDGF-BB and bFGF increased constantly for about 9 days. The release of PDGF-BB and bFGF linearly increased with the amount of PC added in CPC. According to 12 hr culture, the adhered Saos-2 cells increased with the amount of incorporated PC.