Cell Compatibility of Calcium Phosphate Cement Incorporated with Platelet Concentrate

We have recently demonstrated the slow release of growth factors from calcium phosphate cements (CPCs) incorporated with platelet concentrate (PC). In the present study, the cell compatibility of CPCs was evaluated by culturing human osteoblast-like cells Saos-2 (ATCC HTB85) to demonstrate the PC effect. CPCs were characterized using scanning electron microscopy (SEM), X-Ray diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDS) and their mechanical properties were tested. The physical and chemical properties of CPCs were little affected by the PC incorporation within the experimented range. The cell proliferation, morphology and migration, measurement of alkaline phosphatase (ALP) levels, mRNA expression for osteopontin (OPG), osteocalcin (OC), glyceraldehyde phosphate dehydrogenase (GAPDH) were evaluated. Higher cell proliferation was performed but the cell migration was not affected by the addition of PC. Actin filaments, focal adhesion, and localized integrin were clearly visible. The PC addition increased ALP activity and expression of GAPDH, OC, and OPG mRNA.