Production of Penicillin G acylase from Bacillus sp.: Effect of medium components

被引：0
|
作者：
J. Rajendhran
V. Krishnakumar
P. Gunasekaran
机构：
[1] Madurai Kamaraj University,Department of Microbial Technology, Centre for Excellence in Genomic Sciences, School of Biological Sciences
来源：
World Journal of Microbiology and Biotechnology | 2003年 / 19卷
关键词：
sp.; carbon source; intracellular enzyme; nitrogen source; penicillin G acylase;
D O I：
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中图分类号：
学科分类号：
摘要：
A Bacillus sp. producing a high level of intracellular penicillin G acylase (PAC) was isolated. The PAC production in this strain was induced by phenylacetic acid. Various carbon and nitrogen sources were evaluated for their effect on growth and PAC production at 28 °C and pH 7.0. Cells grown in medium supplemented with sucrose as carbon source and tryptone as nitrogen source produced maximum activity of 6.45 and 8.92 U mg−1, respectively. Maximum concentration of PAC (10.1 Umg−1) was produced by the cells grown in the medium containing sucrose and tryptone, which was twofold higher than the production in basal medium.
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页码：107 / 110
页数：3
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