Study of different media for production of penicillin G acylase from Bacillus megaterium ATCC 14945

被引：0
|
作者：
Laura M. Pinotti
Astrea F. S. Silva
Rosineide G. Silva
Raquel L. C. Giordano
机构：
[1] Universidade Federal de São Carlos,Departamento de Engenharia Química
来源：
Applied Biochemistry and Biotechnology | 2000年 / 84-86卷
关键词：
penicillin G acylase; fermentation; medium optimization; enzymatic casein hydrolysates; cheese whey;
D O I：
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学科分类号：
摘要：
In this study, several fermentation media were tested for the production of penicillin G acylase (PGA) using Bacillus megaterium. The carbon sources studied were glucose and lactose. The nitrogen sources studied were enzymatic casein hydrolysates produced with proteases of different specificities. The replacement of glucose with cheese whey and the addition of free amino acids in the PGA production were also tested. The results showed a strong correlation between the nitrogen source and enzyme yield and the presence of glucose repression. The highest enzyme concentration achieved was 138 IU/L using casein hydrolyzed with 0.6 L of Alcalase® and cheese whey.
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页码：655 / 663
页数：8
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