CLONING AND SEQUENCING OF THE PAC GENE ENCODING THE PENICILLIN-G ACYLASE OF BACILLUS-MEGATERIUM ATCC-14945

被引：39
|
作者：
MARTIN, L [1 ]
PRIETO, MA [1 ]
CORTES, E [1 ]
GARCIA, JL [1 ]
机构：
[1] CSIC,CTR INVEST BIOL,DEPT MOLEC MICROBIOL,E-28006 MADRID,SPAIN
来源：
FEMS MICROBIOLOGY LETTERS | 1995年 / 125卷 / 2-3期
关键词：
PENICILLIN ACYLASE; BACILLUS MEGATERIUM; ESCHERICHIA COLI; PAC GENE;
D O I：
10.1016/0378-1097(94)00510-X
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The pac gene encoding the penicillin G acylase (PGA) of Bacillus megaterium ATCC 14945 has been cloned in Escherichia coli HB101 (proA, leuB) using a selective minimal medium containing phenylacetyl-L-leucine instead of L-leucine. The nucleotide sequence of this gene has been determined and contains an open reading frame of 2406 nucleotides. The deduced amino acid sequence shows significant similarity with other beta-lactam acylases. Although the PGA of B. megaterium is extracellular, the enzyme produced in E. coli appears to have a cytoplasmic localization.
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页码：287 / 292
页数：6
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