Production of penicillin G acylase from Bacillus sp.:: Effect of medium components

被引：10
|
作者：
Rajendhran, J [1 ]
Krishnakumar, V [1 ]
Gunasekaran, P [1 ]
机构：
[1] Madurai Kamaraj Univ, Sch Biol Sci, Dept Microbial Technol, Ctr Excellence Genom Sci, Madurai 625021, Tamil Nadu, India
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2003年 / 19卷 / 01期
关键词：
Bacillus sp; carbon source; intracellular enzyme; nitrogen source; penicillin G acylase;
D O I：
10.1023/A:1022512217095
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A Bacillus sp. producing a high level of intracellular penicillin G acylase (PAC) was isolated. The PAC production in this strain was induced by phenylacetic acid. Various carbon and nitrogen sources were evaluated for their effect on growth and PAC production at 28 degreesC and pH 7.0. Cells grown in medium supplemented with sucrose as carbon source and tryptone as nitrogen source produced maximum activity of 6.45 and 8.92 U mg(-1), respectively. Maximum concentration of PAC (10.1 Umg(-1)) was produced by the cells grown in the medium containing sucrose and tryptone, which was twofold higher than the production in basal medium.
引用
收藏
页码：107 / 110
页数：4
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