Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles

被引：0
|
作者：
Yu. A. Romanov
E. E. Balashova
N. E. Volgina
N. V. Kabaeva
T. N. Dugina
G. T. Sukhikh
机构：
[1] Ministry of Health of the Russian Federation,Russian Cardiology Research
[2] Ministry of Health of the Russian Federation,and
[3] CryoCenter Umbilical Cord Blood Bank,Production Complex
来源：
Bulletin of Experimental Biology and Medicine | 2016年 / 160卷
关键词：
umbilical blood; hematopoietic stem cell; cryogenic storage; repeated freezing/thawing cycles; viability;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.
引用
收藏
页码：571 / 574
页数：3
相关论文
共 50 条
  • [31] Stem cell factor improves SCID-repopulating activity of human umbilical cord blood-derived hematopoietic stem/progenitor cells in xenotransplanted NOD/SCID mouse model
    Zheng, Y
    Sun, A
    Han, ZC
    BONE MARROW TRANSPLANTATION, 2005, 35 (02) : 137 - 142
  • [32] Stem cell factor improves SCID-repopulating activity of human umbilical cord blood-derived hematopoietic stem/progenitor cells in xenotransplanted NOD/SCID mouse model
    Y Zheng
    A Sun
    Z C Han
    Bone Marrow Transplantation, 2005, 35 : 137 - 142
  • [33] Dendritic cell differentiation from CD34+hematopoietic stem cells with interferon-γ use in human umbilical cord blood
    Jung, Min Hyung
    Kim, Yong Man
    Song, Ha Young
    Yang, Hyun Ok
    Kim, Jong Hyeok
    Kim, Young Tak
    Mok, Jung Eun
    Nam, Joo Hyun
    CANCER RESEARCH, 2006, 66 (08)
  • [34] Differences in lymphocyte developmental potential between human embryonic stem cell and umbilical cord blood-derived hematopoietic progenitor cells
    Martin, Colin H.
    Woll, Petter S.
    Ni, Zhenya
    Zuniga-Pflucker, Juan Carlos
    Kaufman, Dan S.
    BLOOD, 2008, 112 (07) : 2730 - 2737
  • [35] Towards optimal culture conditions for the ex-vivo expansion of umbilical cord blood hematopoietic stem/progenitor cells for cell therapy
    Andrade, Pedro Z.
    dos Santos, Francisco
    Almeida-Porada, Graca
    da Silva, Claudia Lobato
    Cabral, Joaquim M. S.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012, 243
  • [36] Repeated injections of human umbilical cord blood-derived mesenchymal stem cells significantly promotes functional recovery in rabbits with spinal cord injury of two noncontinuous segments
    Chaohua Yang
    Gaoju Wang
    Fenfen Ma
    Baoqing Yu
    Fancheng Chen
    Jin Yang
    Jianjun Feng
    Qing Wang
    Stem Cell Research & Therapy, 9
  • [37] Hematopoietic stem cell transplantation (HSCT) for benign indications using umbilical cord blood units (UCB) that were depleted of plasma, but not of red blood cells.
    Chow, R
    Jaing, TH
    Gjertson, D
    Rosenthal, J
    Nademanee, A
    Karanes, C
    Graham, M
    Tan, P
    Petz, LD
    BLOOD, 2005, 106 (11) : 451B - 452B
  • [38] Repeated injections of human umbilical cord blood-derived mesenchymal stem cells significantly promotes functional recovery in rabbits with spinal cord injury of two noncontinuous segments
    Yang, Chaohua
    Wang, Gaoju
    Ma, Fenfen
    Yu, Baoqing
    Chen, Fancheng
    Yang, Jin
    Feng, Jianjun
    Wang, Qing
    STEM CELL RESEARCH & THERAPY, 2018, 9
  • [39] Comparison of Maturation Stages of Natural Killer Cell Differentiation Culture from Cultured and Freshly Isolated Umbilical Cord Blood Hematopoietic Stem Cells
    Wijaya, Samuel Febrian
    Lestari, Retno
    Rahmawati, Inna
    Sianipar, Imelda Rosalyn
    Nuraditya, Robby
    Fasha, Iqbal
    Pratama, Gita
    Antarianto, Radiana Dhewayani
    MAKARA JOURNAL OF HEALTH RESEARCH, 2023, 27 (01): : 68 - 76
  • [40] Functional Test and Characteristic of Hematopoietic Stem Cells Derived from Human Umbilical Cord Blood Using Unpad-Aster's Modified Method
    Djuwantono, Tono
    Wirakusumah, Firman F.
    Septiani, Leri
    Kristina, Ike
    Natalia, Devi
    Halim, Danny
    Faried, Ahmad
    MAJALAH KEDOKTERAN BANDUNG-MKB-BANDUNG MEDICAL JOURNAL, 2011, 43 (04): : 171 - 177
12345