Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles

被引：0
|
作者：
Yu. A. Romanov
E. E. Balashova
N. E. Volgina
N. V. Kabaeva
T. N. Dugina
G. T. Sukhikh
机构：
[1] Ministry of Health of the Russian Federation,Russian Cardiology Research
[2] Ministry of Health of the Russian Federation,and
[3] CryoCenter Umbilical Cord Blood Bank,Production Complex
来源：
Bulletin of Experimental Biology and Medicine | 2016年 / 160卷
关键词：
umbilical blood; hematopoietic stem cell; cryogenic storage; repeated freezing/thawing cycles; viability;
D O I：
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中图分类号：
学科分类号：
摘要：
We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.
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页码：571 / 574
页数：3
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