Fast genomic μChIP-chip from 1,000 cells

被引：0
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作者：
John Arne Dahl
Andrew H Reiner
Philippe Collas
机构：
[1] Faculty of Medicine,Institute of Basic Medical Sciences, Department of Biochemistry
[2] University of Oslo,undefined
来源：
Genome Biology | / 10卷
关键词：
Transcription Start Site; Additional Data File; Catalogue Number; Whole Genome Amplification; Qubit Fluorometer;
D O I：
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摘要：
Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (μChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. μChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.
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