Polyaniline based catalase biosensor for the detection of hydrogen peroxide and azide

被引：0
|
作者：
Ravindra P. Singh
Da-Yeon Kang
Byung-Keun Oh
Jeong-Woo Choi
机构：
[1] Sogang University,Department of Chemical and Biomolecular Engineering
[2] Sogang University,Interdisciplinary Program of Integrated Biotechnology
来源：
Biotechnology and Bioprocess Engineering | 2009年 / 14卷
关键词：
Polyaniline (PANi); catalase (CAT); hydrogen peroxide (H; O; ); azide; biosensor; bioelectrode;
D O I：
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中图分类号：
学科分类号：
摘要：
The CAT/PANi/ITO bioelectrode has been prepared as a catalase biosensor and shows response for monitoring not only of H2O2 but also azide. The sensor exhibited an excellent response to the H2O2 and azide. The linear range of H2O2 was 0.064∼1 mM and for azide 0.125∼4 mM, respectively. Catalase biosensor was based on the principle of the measurements as the decrease in the differentiation of oxygen level, which has been caused by the inhibition of catalase in the bioactive layer of the biosensor by azide. The repeatability experiments were done in triplicate. The logarithm response of the biosensor to H2O2 (r2 = 0.99), as well as, for azide (r2 = 0.90) were reported, respectively. The bioelectrode was characterized by CV and AFM. The proposed biosensor would be applied for the determination of H2O2 and azide in various biological samples.
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页码：443 / 449
页数：6
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