BIOSENSOR FOR DETERMINATION OF HYDROGEN-PEROXIDE BASED ON CATALASE ACTIVITY OF HUMAN ERYTHROCYTES

A hydrogen peroxide biosensor based on human erythrocytes is described. Erythrocytes are retained on the surface of an oxygen electrode by a semipermeable membrane. The response is based on the catalase activity of the erythrocytes. The sensitivity of 10-4 mol 1-1 and linearity from 1.5×10-4 to 5×10-3 mol-1 are comparable to those of analogous enzyme biosensors for hydrogen peroxide determination. The greatest advantages of this biosensor are its easy preparation and a lifetime of 2 months together with good reproducibility (relative standard deviation <5%) and selectivity; only ascorbic acid appeared to interfere with the measurements. © 1990.