BIOSENSOR FOR DETERMINATION OF HYDROGEN-PEROXIDE BASED ON CATALASE ACTIVITY OF HUMAN ERYTHROCYTES

被引：10
|
作者：
RACEK, J
PETR, R
机构：
[1] Department of Clinical Biochemistry, University Hospital
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 239卷 / 01期
关键词：
Biosensor; Enzyme electrode; Hydrogen peroxide;
D O I：
10.1016/S0003-2670(00)83830-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A hydrogen peroxide biosensor based on human erythrocytes is described. Erythrocytes are retained on the surface of an oxygen electrode by a semipermeable membrane. The response is based on the catalase activity of the erythrocytes. The sensitivity of 10-4 mol 1-1 and linearity from 1.5×10-4 to 5×10-3 mol-1 are comparable to those of analogous enzyme biosensors for hydrogen peroxide determination. The greatest advantages of this biosensor are its easy preparation and a lifetime of 2 months together with good reproducibility (relative standard deviation <5%) and selectivity; only ascorbic acid appeared to interfere with the measurements. © 1990.
引用
收藏
页码：19 / 22
页数：4
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