Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells

被引：0
|
作者：
Wusheng Liu
Mary R. Rudis
Matthew H. Cheplick
Reginald J. Millwood
Jian-Ping Yang
Christine A. Ondzighi-Assoume
Garrett A. Montgomery
Kellie P. Burris
Mitra Mazarei
Jonathan D. Chesnut
Charles Neal Stewart
机构：
[1] University of Tennessee,Department of Plant Sciences
[2] North Caroline State University,Department of Horticultural Science
[3] Thermo Fisher Scientific,Synthetic Biology Research and Development
[4] Tennessee State University,Department of Agricultural and Environmental Sciences
[5] North Caroline State University,Department of Food, Bioprocessing and Nutrition Sciences
[6] University of Tennessee Institute of Agriculture,Center for Agricultural Synthetic Biology
来源：
Plant Cell Reports | 2020年 / 39卷
关键词：
Protein delivery; Cas9/gRNA ribonucleoprotein; Lipofection; Biolistics; Genome editing; Protoplast; Tobacco; BY2;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
引用
收藏
页码：245 / 257
页数：12
相关论文
共 50 条
  • [41] Efficient DNA-free nanoparticle mediated genome editing of potato using CRISPR-Cas9 RNP complex
    Khromov, A.
    Makarov, V.
    Makhotenko, A.
    Makarova, S.
    Snigir, E. A.
    Suprunova, T. P.
    Kalinina, N. O.
    Taliansky, M. E.
    FEBS OPEN BIO, 2018, 8 : 167 - 167
  • [42] DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
    Je Wook Woo
    Jungeun Kim
    Soon Il Kwon
    Claudia Corvalán
    Seung Woo Cho
    Hyeran Kim
    Sang-Gyu Kim
    Sang-Tae Kim
    Sunghwa Choe
    Jin-Soo Kim
    Nature Biotechnology, 2015, 33 : 1162 - 1164
  • [43] CRISPR/Cpf1-mediated DNA-free plant genome editing
    Kim, Hyeran
    Kim, Sang-Tae
    Ryu, Jahee
    Kang, Beum-Chang
    Kim, Jin-Soo
    Kim, Sang-Gyu
    NATURE COMMUNICATIONS, 2017, 8
  • [44] Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
    Brett T Staahl
    Madhurima Benekareddy
    Claire Coulon-Bainier
    Ashwin A Banfal
    Stephen N Floor
    Jennifer K Sabo
    Cole Urnes
    Gabriela Acevedo Munares
    Anirvan Ghosh
    Jennifer A Doudna
    Nature Biotechnology, 2017, 35 : 431 - 434
  • [45] Lentiviral Capsid-Mediated Streptococcus pyogenes Cas9 Ribonucleoprotein Delivery for Efficient and Safe Multiplex Genome Editing
    Lu, Zuyan
    Yao, Xingang
    Lyu, Pin
    Yadav, Manish
    Yoo, Kyung
    Atala, Anthony
    Lu, Baisong
    CRISPR JOURNAL, 2021, 4 (06): : 914 - 928
  • [46] DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
    Woo, Je Wook
    Kim, Jungeun
    Kwon, Soon Il
    Corvalan, Claudia
    Cho, Seung Woo
    Kim, Hyeran
    Kim, Sang-Gyu
    Kim, Sang-Tae
    Choe, Sunghwa
    Kim, Jin-Soo
    NATURE BIOTECHNOLOGY, 2015, 33 (11) : 1162 - +
  • [47] Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells
    Liu, Qizhi
    Yuan, Yongming
    Zhu, Feng
    Hong, Yunhan
    Ge, Ruowen
    BIOLOGY OPEN, 2018, 7 (08):
  • [48] Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
    Staahl, Brett T.
    Benekareddy, Madhurima
    Coulon-Bainier, Claire
    Banfal, Ashwin A.
    Floor, Stephen N.
    Sabo, Jennifer K.
    Urnes, Cole
    Munares, Gabriela Acevedo
    Ghosh, Anirvan
    Doudna, Jennifer A.
    NATURE BIOTECHNOLOGY, 2017, 35 (05) : 431 - +
  • [49] CRISPR/Cpf1-mediated DNA-free plant genome editing
    Hyeran Kim
    Sang-Tae Kim
    Jahee Ryu
    Beum-Chang Kang
    Jin-Soo Kim
    Sang-Gyu Kim
    Nature Communications, 8
  • [50] DNA-free mutagenesis of GIGANTEA in Brassica oleracea var. capitata using CRISPR/Cas9 ribonucleoprotein complexes
    Park, Sung-Chul
    Park, Suhyun
    Jeong, Yu Jeong
    Lee, Saet Buyl
    Pyun, Jang Won
    Kim, Soyoung
    Kim, Tae Hee
    Kim, Suk Weon
    Jeong, Jae Cheol
    Kim, Cha Young
    PLANT BIOTECHNOLOGY REPORTS, 2019, 13 (05) : 483 - 489
12345