Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells

被引:0
|
作者
Wusheng Liu
Mary R. Rudis
Matthew H. Cheplick
Reginald J. Millwood
Jian-Ping Yang
Christine A. Ondzighi-Assoume
Garrett A. Montgomery
Kellie P. Burris
Mitra Mazarei
Jonathan D. Chesnut
Charles Neal Stewart
机构
[1] University of Tennessee,Department of Plant Sciences
[2] North Caroline State University,Department of Horticultural Science
[3] Thermo Fisher Scientific,Synthetic Biology Research and Development
[4] Tennessee State University,Department of Agricultural and Environmental Sciences
[5] North Caroline State University,Department of Food, Bioprocessing and Nutrition Sciences
[6] University of Tennessee Institute of Agriculture,Center for Agricultural Synthetic Biology
来源
Plant Cell Reports | 2020年 / 39卷
关键词
Protein delivery; Cas9/gRNA ribonucleoprotein; Lipofection; Biolistics; Genome editing; Protoplast; Tobacco; BY2;
D O I
暂无
中图分类号
学科分类号
摘要
引用
收藏
页码:245 / 257
页数:12
相关论文
共 50 条
  • [11] Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing
    Lee, Sora
    Park, Su Hyun
    Jeong, Yu Jeong
    Kim, Soyoung
    Kim, Bo Ryeong
    Ha, Bo-Keun
    Na, Chaein
    Lee, Jiyoung
    Jeong, Jae Cheol
    Kim, Cha Young
    PLANT BIOTECHNOLOGY REPORTS, 2024, 18 (03) : 415 - 424
  • [12] Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
    Xiaonan Ma
    Xiaoyan Zhang
    Huimin Liu
    Zhenghe Li
    Nature Plants, 2020, 6 : 773 - 779
  • [13] Genome editing via delivery of Cas9 ribonucleoprotein
    DeWitt, Mark A.
    Corn, Jacob E.
    Carroll, Dana
    METHODS, 2017, 121 : 9 - 15
  • [14] Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
    Zhang, Song
    Shen, Jiangtao
    Li, Dali
    Cheng, Yiyun
    THERANOSTICS, 2021, 11 (02): : 614 - 648
  • [15] DNA-free genome editing with preassembled CRISPR/Cas9 ribonucleoproteins in plants
    Park, Jongjin
    Choe, Sunghwa
    TRANSGENIC RESEARCH, 2019, 28 (Suppl 2) : 61 - 64
  • [16] DNA-free genome editing with preassembled CRISPR/Cas9 ribonucleoproteins in plants
    Jongjin Park
    Sunghwa Choe
    Transgenic Research, 2019, 28 : 61 - 64
  • [17] Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
    Lin, Qingyi
    Le, Quynh Anh
    Takebayashi, Koki
    Thongkittidilok, Chommanart
    Wittayarat, Manita
    Hirata, Maki
    Tanihara, Fuminori
    Otoi, Takeshige
    BMC RESEARCH NOTES, 2021, 14 (01)
  • [18] Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events
    Qingyi Lin
    Quynh Anh Le
    Koki Takebayashi
    Chommanart Thongkittidilok
    Manita Wittayarat
    Maki Hirata
    Fuminori Tanihara
    Takeshige Otoi
    BMC Research Notes, 14
  • [19] Genome editing in vivo with the delivery of Cas9 ribonucleoprotein and donor DNA complexed to nanoparticles
    Murthy, Niren
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 253
  • [20] Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
    Liang, Xiquan
    Potter, Jason
    Kumar, Shantanu
    Ravinder, Namritha
    Chesnut, Jonathan D.
    JOURNAL OF BIOTECHNOLOGY, 2017, 241 : 136 - 146