Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags

被引：0
|
作者：
Onisha Patel
Ross Fernley
Ian Macreadie
机构：
[1] CSIRO Health Sciences and Nutrition,
[2] RMIT University,undefined
来源：
Biotechnology Letters | 2003年 / 25卷
关键词：
dihydrofolate synthase; hexahistidine tags; protein purification; thrombin cleavage; yeast expression system;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
General-purpose yeast expression vectors for convenient cloning and production of proteins with N- or C-terminal His6 tags that can be efficiently removed with thrombin have been developed. To the parental yeast-E. coli shuttle vectors that have convenient copper-inducible expression, two selectable markers and LEU2d vector amplification, this development adds substantial versatility to product recovery.
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页码：331 / 334
页数：3
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