Saccharomyces cerevisiae expression vectors with thrombin-cleavable N- and C-terminal 6x(His) tags

被引：0

作者：

Onisha Patel

Ross Fernley

Ian Macreadie

机构：

[1] CSIRO Health Sciences and Nutrition,

[2] RMIT University,undefined

来源：

Biotechnology Letters | 2003年 / 25卷

关键词：

dihydrofolate synthase; hexahistidine tags; protein purification; thrombin cleavage; yeast expression system;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

General-purpose yeast expression vectors for convenient cloning and production of proteins with N- or C-terminal His6 tags that can be efficiently removed with thrombin have been developed. To the parental yeast-E. coli shuttle vectors that have convenient copper-inducible expression, two selectable markers and LEU2d vector amplification, this development adds substantial versatility to product recovery.

引用

页码：331 / 334

页数：3

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