A protocol for islet isolation from mouse pancreas

被引：0
|
作者：
Dong-Sheng Li
Ya-Hong Yuan
Han-Jun Tu
Qing-Le Liang
Long-Jun Dai
机构：
[1] Hubei Key Laboratory of Embryonic Stem Cell Research,Department of Surgery
[2] Tai-He Hospital,undefined
[3] Yunyang Medical College,undefined
[4] Shiyan,undefined
[5] Ike Barber Human Islet Transplant Laboratory,undefined
[6] University of British Columbia,undefined
来源：
Nature Protocols | 2009年 / 4卷
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摘要：
Mouse islets are commonly used in diabetes-related studies. Adequate amounts of good quality islets are prerequisites for a reliable investigation. We describe a protocol for islet isolation from mouse pancreas. Three major manipulations are employed in the islet isolation procedure: in situ pancreas perfusion with collagenase, pancreas digestion and islet purification. The whole procedure takes 30–45 min for each individual mouse. By using this protocol, a reasonable number of islets can be obtained in a relatively short period of time. This protocol has been proven to be practicable and reproducible. It can be easily followed by individuals who do not have previous experience in the related research field.
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页码：1649 / 1652
页数：3
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